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Carolina State University School of Veterinary Medicine in 1988 when fish cultures in the research laboratory died from contamination by Pfiesteria.
A botanist, Jane Burkholder, Was called in to help and she happened to havfe a background in dinoflagellates, an unusual group of organisms of which Pfiesteria is a member. She recognized it as' an unidentified organism.
Unfortunately, she had no idea of the organism’s toxicity and she did not take the precautions that are now mandatory when working with it.
Research on Pfiesteria in the toxic stage now requires a Class 3 laboratory (as used with the Ebola virus) and research workers must wear special protective suits.
She grew massive populations of the organism in fish tanks in the laboratory and air contamination occurred from the tanks’ air bubbles. She and her assistant suffered irreversible respiratory problems and temporary cognitive problems including the assistant’s loss of memory which left him with a third-grade reading level. He had to go -through Hie process of relearning how to read beyond that level.
Burkholder named the organism after Lois Pfiester, her mqjor instructor in graduate school and the one who instruct
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